Fungal screening and phylogenetic tree construction

The second-generation sequencing technology has achieved rapid development in recent years. It has the advantages of fast speed, high accuracy and low cost compared to the former sequence, so it has a widely application. Marine fungi are important sources of active Marine natural products. Due to the differences in the thermodynamic properties of land and sea and the concentration of salt oxygen, it has a great potential to produce new bioactive substances. Nowadays, scientists isolate and identify a large number of secondary metabolites with novel structures which is from marine fungi, and these compounds show excellent biological activities. For example, some are potential drugs to treat cancer. In order to search for new fungi, this article used a series of biological methods to isolate and study the bacteria isolated from huimin wharf and determine their specific species. And this paper also uses 18S rDNA to analyse the gene sequence and construe the phylogenetic tree.