Production of IL-10 and IL-12 by AM and PBMC from lung cancer patients

Objective:To investigate the production of IL-10 and IL-12 by alveolar marcrophage and peripheral blood mononuclear cell from lung cancer patients,to evaluate the cellular immune state,and to provide justification for IL-12 therapy in the fields of Lung cancer.Methods:AM and PBMC were obtained from 57 patients with Lung cancer,33 patients with benign pulmonary disease,and 12 normal control.IL-10 and IL-12 in the culture supernatants were measured quantitatively by ELISA.Results:(1) Higher levels of IL-10 were found in Lung cancer group than that in NC and BPD group.IL-12 showed significantly lower levels than that in NC and BPD group.(2) The levels of IL-10 had negative correlation with that of IL-12 in Lung cancer group.(3) The levels of IL-10 in Lung cancer group of stage Ⅲ,Ⅳ were significantly higher than that of stage Ⅰ～Ⅱ.The levels of IL-12 in Lung cancer group of stage Ⅳ were significantly lower than that of stage Ⅰ～Ⅱ.(4) Higher levels of IL-10 were found in SCLC group than that in NSCLC group.The levels of IL-12 from PBMC and AM in SCLC group were lower than that in adenocarcinoma,adenocarcinoma and squamous carcinoma groups separately.Conclusion:(1) results suggest the defect of cell-mediated immunity function presents both in local and general immune response in Lung cancer patients.(2)The basal levels of IL-10 and IL-12 are related to the stage and extent of malignancy.