Analysis of the experimental influences on the procedure of cDNA microarray

Objective:To analyze the experimental influences on the procedures of cDNA microarray designed for different expression of apoptosis and signal transduction and to establish and optimize the method for further clinical application.Methods:Total RNA of mouse liver tissue was extracted, cDNA probes were labelled with fluorescence by means of reverse transcription,hybridized to cDNA microarray,and then washed and desiccated.Hybridization signals were scanned and recorded.Results:High quality total RNA was acquired;reaction efficiency of reverse transcription was evaluated;hybridization signals of DNA microarray was scanned and detected clearly.Conclusions:High quality total RNA and reaction efficiency of reverse transcription are crucial to cDNA microarray technology.