Molecular identification and acquisition of interacting partners of a novel wheat F-box/Kelch gene TaFBK

Abstract F-box proteins constitute one of the largest protein families and play many important roles in plant. To date, less is known about functions of wheat F-box protein. A Kelch-type F-box gene TaFBK was isolated from wheat TcLr15. TaFBK transcripts were more abundant in pistil and leaf tissues, and accumulated more in the susceptible combination than that in the resistant one. The treatment of SA, MeJA, NaCl and PEG caused a slight up-regulation of TaFBK. GFP localization studies showed that TaFBK accumulates in the nucleus and cytoplasm. Moreover, TaFBK bound TaSkp1 by the F-box domain and recognized TaSLY1 via the Kelch domain.