Method for extracting cordycepin from cordyceps militaris rice medium under ultrahigh pressure
2014
The invention relates to a method for extracting cordycepin from a cordyceps militaris rice medium under ultrahigh pressure, and belongs to the technical field of natural products. The technological process of the method comprises the following steps: performing slice on the cordyceps militaris rice medium, drying with hot air, crushing, extracting under ultrahigh pressure, performing centrifugation, removing impurities through a ceramic membrane, concentrating, freeze-drying, purifying cordycepin through a high-speed countercurrent chromatography, and detecting through a high performance liquid chromatography. According the method, the yield of the cordycepin from the cordyceps militaris rice medium is 1.2 times that of a common method, and the purity can reach more than 90%. The method adopts ultrahigh pressure to extract the cordycepin, so that advantages of high extraction rate, low impurity content, good stability and the like are realized; high temperature is prevented in the whole extraction process, so that active ingredients and structures of the cordycepin can be effectively retained. The cordycepin prepared by the method is safe and free from contamination, has a high food (drug) use value, and provides a certain market application prospect and economic benefits for comprehensive utilization of edible fungus cordyceps militaris by-products.
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