Protect effects of Qingkailing injection on mitochondrion membrane potential during injury induced by hypoxia-hypoglycemia and reoxygenation in cultured rat hippocampal neurons

Objective: To investigate the protect effects of Qingkailing injection on mitochondrion membrane potential(MMP) during injury induced by hypoxia-hypoglycemia and reoxygenation in cultured rat hippocampal neurons.Method: Mitochondrion activity was measured by methyl thiazolyl tetrazolium(MTT)test.MMP and apoptosis were measured by flow cytometry.intracellular free calcium concentration([Ca~(2+)]i)was measured with confocal laser scanning microscopy.Result: Hypoxia-hypoglycemia cultures for 5 hour and reoxygenation for 3 hour induced intracellular[Ca~(2+)]i and apoptosis rate significantly increased.The effects were increased with the extending time of reoxygenation.MMP and mitochondrion activity declined significantly after 3 hour reoxygenation.The effects were declined with the extending time of reoxygenation.Qingkailing injection might have significantly decrease intracellular [Ca~(2+)]i and apoptosis rate,increase MMP and mitochondrion activity.Conclusion: Qingkailing Injection might have significantly inhibit the decline in MMP induced by hypoxia-hypoglycemia and reoxygenation,and had effects of stable it and anti-neuronal apoptosis.The effects might be related to inhibit overload of intracellular free calcium.