Cytotoxic terpenoids from Juglans sinensis leaves and twigs.

Abstract Bioassay-guided fractionation of an 80% MeOH extract of Juglan sinensis leaves and twigs has resulted in the isolation of three new triterpenes ( 1 – 3 ) and two new sesquiterpenes ( 4 – 5 ) along with two known sesquiterpenes ( 6 – 7 ). The new compounds were determined to be 3β, 11α, 19α, 24, 30-pentahydroxy-20β, 28-epoxy-28β-methoxy-ursane ( 1 ), 1α, 3β-dihydroxy-olean-18-ene ( 2 ), 2α, 3α, 23-trihydroxy-urs-12-en-28-oic acid 28- O -β- d -glucopyranoside ( 3 ), (4 S , 5 S , 7 R , 8 R , 14 R )-8, 11-dihydroxy-2, 4-cyclo-eudesmane ( 4 ), 15-hydroxy-α-eudesmol-11- O -β- d -glucopyranoside ( 5 ), by spectroscopic analysis. The cytotoxicity of compounds ( 1 – 7 ) against four cancer cell lines such as B16F10, Hep-2, MCF-7 and U87-MG was evaluated. Compounds 1 , 2 , 6 and 7 showed potent cytotoxicity against all of four cancer cell lines, respectively.