Molecular cloning and sequence analysis of human activation-inducible tumor necrosis factor recepter ligand gene

Objective: To clone and analyze a full-lenth cDNA encoding human activation-inducible tumor necrosis factor receptor ligand(AITRL) gene.Methods: The cDNA of AITRL was amplified by RT-PCR using the total RNA extracted from human umbilical vein endothelial cell(HUVEC).The PCR pro-duct was inserted into pMD18-T vector and then transformed into E.coli DH5α.The positive recombinant clone was analyzed by digestion of restriction endonuclease and DNA sequencing. Results: The cDNA of AITRL has an complete open reading frame with a length of 534 bp,which encodes a product of 177 amino acid,and shares 100 % homology with the sequence of mRNA for human AITRL in GenBank. Conclusion: The cDNA of human AITRL was cloned successfully, which posed a basis for further researching on its biological function.