2P-244 : Photodynamic Therapy for the Treatment of Pancreatic Cancer Using Chlorin e6 loaded Exosome

Exosome, extracellular vesicles for cell-to-cell communications, has been utilized as drug delivery system. In this study, tumor-derived exosome was sonicated and reassembled for a drug delivery carrier. Chlorin e6 (Ce6), a representative photosensitizer, was loaded into tumor-derived exosome during re-assembly of exosomes. The reassembled exosomes was found to have membrane proteins like the exosomes. By removing the internal substances, cancer formation from tumor-derived exosomes could be resolved. In vitro experimental data showed that Ce6-loaded re-assembled exosomes were selectively targeted to original-derived cells. Due to the high specific targeting ability of exosome, the Ce6 could be effectively delivered. Furthermore, after effective intracellular delivery, sufficient reactive oxygen species (ROS) were generated with very small amounts of Ce6 in the target cells under the laser irradiation. In conclusion, photodynamic therapy could be obtained with Ce6- loaded exosome.