Effects of oxidising atmosphere on brewer’s yeast hydrothermal treatment and subsequent biopolymer recovery
2020
Abstract Spent breweŕs yeast is an underused by-product, whose current management offers low returns. This article studies, for the first time ever, the hydrothermal treatment of spent breweŕs yeast, paying special attention to the effect of the presence of oxygen on the physical properties, composition and potential recovery of valuable products from the hydrolysed yeast, as previous step towards the employment of these recovered proteins as renewable chemical feedstock for green industrial uses. Results showed a higher VSS disintegration employing an oxidising atmosphere, as well as a better solubilisation in terms of solid COD reduction, although it also lowered soluble COD but without significantly reducing soluble TOC. Both treatments led to an almost complete solubilisation of protein and carbohydrates from sludge, which were subsequently degraded in presence of oxygen if reaction times were lengthened. Looking at biopolymer recovery, protein precipitation by pH adjustment worked better in hydrolysates obtained in presence of oxygen, with almost 90% of protein precipitation and good selectivities at pH 2.5 and 3. Salting out was more effective, in terms of selectivity, for samples obtained in absence of oxygen and low concentrations of ammonium sulphate. The application of IMAC showed better results on yeast hydrolysates obtained under an inert atmosphere, reaching maximum sorption capacities of almost 100 mg protein/ g dry resin, three times higher than those observed using the hydrolysate produced in presence of oxygen. A new perspective in yeast management during beer production is open as renewable chemical feedstock for protein, although further studies are required to optimise protein recovery and purification with respect to its applications.
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