[Expression and significance of PDK1 and NF-κB in the middle ear cholesteatoma].

: Objective:To detect the expression of 3-phosphoinositide-dependent kinase-1(PDK1) and nuclear factor-κB(NF-κB) in human middle ear cholesteatoma tissue and to analyze their correlation.Method:Immunohistochemical method was taken to detect the expression and location of PDK1 and NF-κB in 60 cases of cholesteatoma tissue and 30 cases of normal ear skin specimens.SPSS 19.0 software was used to analyze the data. Result:Immunohistochemistry revealed PDK1 was positive in cytoplasm and the positive expression rate in cholesteatoma was significantly higher than normal skin (P <0.05); NF-κB expressed in the nucleus of cholesteatoma and the positive expression rate in cholesteatoma significantly higher than normal skin(P <0.05);In cholesteatoma specimens,there was a significant positive correlation between protein PDK1 and NF-κB(P <0.05). Conclusion:Abnormal expression of PDK1 and NF-κB may be related to the proliferation of cholesteatoma epithelium and they reinforce each other.