Proteomic Analysis of Heart and Kidney Tissues in Healthy and Metabolic Syndrome Rats after Hesperidin Supplementation.

SCOPE: Proteomics has provided new strategies to elucidate the mechanistic action of hesperidin, a flavonoid present in citrus fruits. Thus, the aim of the present study was to determine the effects of hesperidin supplementation (HS) on the proteomic profiles of heart and kidney tissue samples from healthy and metabolic syndrome (MS) rats. METHODS AND RESULTS: Twenty-four Sprague-Dawley rats were randomized into 4 groups: healthy rats fed a standard diet without HS, healthy rats administered HS (100 mg/kg/day), MS rats without HS, and MS rats administered HS (100 mg/kg/day) for 8 weeks. Heart and kidney samples were obtained, and proteomic analysis was performed by mass spectrometry. Multivariate, univariate and ingenuity pathways analyses were performed. Comparative and semiquantitative proteomic analyses of heart and kidney tissues revealed differential protein expression between MS rats with and without HS. The top diseases and functions implicated were related to the cardiovascular system, free radical scavenging, lipid metabolism, glucose metabolism and renal and urological diseases. CONCLUSIONS: This study is the first to demonstrate the protective capacity of hesperidin to change to the proteomic profiles in relation to different cardiovascular risk biomarkers in the heart and kidney tissues of MS rats. This article is protected by copyright. All rights reserved.