Cloning and Fusion Expression of Antibacterial Protein Diptericin Gene in Escherichia coli

A cDNA encoding diptericin was extracted from Drosophila adults through RT RCR.The cDNA was cloned into pGEM T vector.After restriction analysis and DNA sequencing,the gene was ligated with fusion expression vector pMAL CR1 and transfected into E.coli BL21.The positive clone was induced by IPTG.The expression product was purified by ion exchange chromatography and digested by factor Xa.The final product,rDiptericin,exhibited antibacterial activity.