Suppression of lymphocyte reactivity in vitro by porcine allantoic and amniotic fluids.

Abstract Porcine allantoic and amniotic fluids from early, mid and late pregnancy were analyzed for protein constitutents and ability to suppress phytohemagglutinin (PHA)-induced lymphocyte transformation. All fetal fluids contained α-fetoprotein as evaluated by polyacrylamide gel electrophoresis (PAGE), with the highest concentrations appearing in mid-pregnancy amniotic fluids. In addition, allantoic fluids from mid-pregnancy contained proteins assumed to be secreted by the uterus. Both allantoic and amniotic fluids from mid-pregnancy were suppressive to PHA-induced lymphocyte transformation (P