Withanolides from Hyoscyamus niger seeds

Three withanolide class steroids were isolated from the seeds of Hyoscyamus niger. Two of them were identified as daturalactone-4 (1) and Nic-3 (which is now named hyoscyamilactol) (2). The new compound was elucidated as 16α-acetoxyhyoscyamilactol (3) on the basis of spectroscopic properties and X-ray crystallographic analysis.