Injectable Oxygen-Generating Nanocomposite Hydrogels with Prolonged Oxygen Delivery for Enhanced Cell Proliferation under Hypoxic and Normoxic Conditions

We describe a new organic peroxide-based injectable biomaterial that was prepared by using Benzoylperoxide and Laponite incorporated alginate hydrogel (BPO-AlgL). BPO-AlgL show sustained release of O2 over a period of 14 days and reduces the hypoxia-induced cell death. BPO-AlgL also promotes enhanced cell viability by providing sustained O2 within the 3D AlgL scaffold. In addition, BPO-AlgL increases the O2 level in the environment of the cells that led a decrease in the proliferation of malignant cells while that resulted in an increase in the viability of healthy fibroblast cells. Therefore, our new oxygen generating organic peroxide-based injectable 3D biomaterials have potential to contribute in the development of the next generation advanced tissue engineering biomaterials.