Prevalence and associated factors associated with Uropathogenic Escherichia coli isolates from catheterized persons at Ilorin Tertiary Hospital, Nigeria

Background and study aim: Urinary tract infection (UTI) is among the most common complications of catheterized persons in hospital settings especially, those caused by extended spectrum beta lactamase (ESBL) and biofilm producing Escherichia coli.This hospital based, cross sectional study was carried out to determine the prevalence and associated factors associated with Uropathogenic Escherichia coli (UPEC) isolates from catheterized persons (inpatients and outpatients) attending Ilorin Tertiary hospital, Nigeria Materials and Methods: Between 2nd April and 30th June 2016, urine samples from 113 catheterized inpatient and outpatients were evaluated. Female subjects accounted for 47(41.6%) of the study population. Standard microbiological methods and Analytical Profile Index (API) 20E system were used for the isolation and identification of UPEC, respectively. Tissue culture plate (TCP) technique was used to demonstrate biofilm production potentials. Results: The prevalence of catheter associated urinary tract infection (CA-UTI) in this study was 70.8% most of which are Gram negative bacilli belonging to the Enterobacteriaceae family with Escherichia coli 44(55.0%) being the most predominant pathogen.Extended spectrum beta lactamase (ESBL) E coli in this study was 27.2% of which all (100%) were strong biofilm producers. Female subjects had relatively higher prevalence of UPEC isolates, 29 (65.9%) than the male counterparts. Whereas, the highest cases of UPEC was in 61-70 years age group, 29.5%. There was significant association between UPEC and age and gender of subjects (p<0.05). Conclusion: Findings from this study shows that E. coli is still the most common uropathogenic bacteria isolate in catheterized persons. Biofilm production confers some degree of ESBL production and antibiotic resistance.