Transient expression of c-Fos in rat amygdala during training is required for encoding conditioned taste aversion memory.

Local m i c r o i n j e c t i o n in to ra t a m y g d a l a o f p h o s p h o r o t h i o a t e m o d i f i e d o l i g o d e o x y n u c l e o t i d e s (ODNs) a n t i s e n s e t o c-los seve ra l h o u r s b e f o r e c o n d i t i o n e d taste a v e r s i o n (CTA) t r a i n i n g i m p a i r e d tas te a v e r s i o n m e m o r y t e s t ed 3 -5 days af ter c o n d i t i o n i n g . In con t ras t , i n j e c t i o n o f t he a n t i s e n s e ODNs severa l days b e f o r e t r a in ing , b e f o r e t es t ing , o r i n to t h e basa l gangl ia , o r i n j e c t i o n o f c-fos s e n s e ODNs, h a d n o effect o n CTA m e m o r y . I n h i b i t i o n o f t r a n s l a t i o n b y loca l m i c r o i n j e c t i o n o f a n i s o m y c i n in to t h e a m y g d a l a s h o r t l y b e f o r e as we l l as d u r i n g CTA t r a i n i ng , b u t n o t severa l days b e f o r e t r a i n i n g o r s h o r t l y b e f o r e tes t ing , a lso i m p a i r e d CTA m e m o r y . We c o n c l u d e t ha t t r a n s l a t i o n in g e n e r a l , a n d c-Fos t r a n s l a t i o n in pa r t i c u l a r , in t h e a m y g d a l a d u r i n g o r i m m e d i a t e l y af ter CTA t r a i n i n g is e s s en t i a l for e n c o d i n g taste a v e r s i o n