Label-free detection of protein-protein interactions on multi-scale micro-well arrays using spatial light modulator

A mass production method of label-free protein microarray integrated with micro-well structures for the use of miniaturized multi-parallel scanning system was investigated. The geometrical parameters of biosensing structure were designed by rigorous coupled wave analysis simulation, and micro-well structures were designed considering the detection and material delivery system. The protein microarray with micro-well structures was fabricated by one-step UV nanoimprinting process using an electroformed multi-scale metallic stamp. Finally, microarray scanning was achieved using optical modulation without applying any motorized system and the feasibility of proposed protein microarray and scanning system was demonstrated by verifying the bio-molecular interactions.