Plant Regeneration from Leaf Protoplasts of Melon (Cucumis melo L.)

Melon leaf protoplasts were successfully regenerated into intact plants in this study. Protoplasts frequently divided and formed callus tissues when 0.1mg/l 2, 4-D and 0.5mg/l BA were added to half strength MS medium containing 0.4M mannitol. Shoot formation was initiated when the callus tissues were transferred to solid MS medium containing 0.03mg/l IAA and 1.0mg/l BA. Shoots elongated roots in the absence of hormone and grew normally under in soil after acclimation. These regenerants produced viable seeds by self-pollenation.