Crystallization and preliminary X-ray diffraction analysis of a cold-adapted catalase from Vibrio salmonicida
2006
Catalase (EC 1.11.1.6) catalyses the breakdown of hydrogen peroxide to water and molecular oxygen. Recombinant Vibrio salmonicida catalase (VSC) possesses typical cold-adapted features, with higher catalytic efficiency, lower thermal stability and a lower temperature optimum than its mesophilic counterpart from Proteus mirabilis. Crystals of VSC were produced by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. The crystals belong to the monoclinic space group P21, with unit-cell parameters a = 98.15, b = 217.76, c = 99.28 A, β = 110.48°. Data were collected to 1.96 A and a molecular-replacement solution was found with eight molecules in the asymmetric unit.
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