Fourier Domain Optical Coherence Microscopy with extended depth of field
2007
Fourier Domain Optical Coherence Tomography (FDOCT) is a high speed biomedical imaging modality which extracts the sample structure in depth. The axial resolution is given by the coherence length of the employed light source. The lateral resolution on the other hand is determined by the numerical aperture (NA) of the objective. The parallel detection of the depth information has the drawback of loosing transverse resolution along the optical axis, limiting the depth of field (DOF) and the use of FDOCT in the field of microscopy. The principle idea to overcome this problem is to illuminate the sample with a cylindrically symmetric interference pattern. Such Bessel beam illumination creates a laterally highly confined needle extending several 100μm along the optical axis in depth.
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