Expressions of LMP-1,p53,Bcl-2 and MMP-9 in Tissues of Lung Cancer with Epstein-Barr Virus Infection

Purpose To investigate the expressions of Epstein-Barr virus (EBV) latent infection membrane protein 1(LMP-1),p53,Bcl-2,matrix matalloproteinases-9(MMP-9) in tissues of lung cancer with EBV infection,and to explore their relationship with carcinogenesis of lung cancer. Methods EBV encoded small RNA-1 (EBER-1) in 108 tissue samples of lung cancer and 22 cases of cancer adjacent tissues were detected by in situ hybridization. The expressions of LMP-1,p53,Bcl-2 and MMP-9 in EBV positive and negative cases were detected immunohistochemically. Results Positive expression of EBER-1 was found in 36 of 108 cases with lung cancer (33.3%), and in 1 of 22 cases of cancer adjacent tissues(4.5%),with significant difference(P0.01). Positive rate of LMP-1 in EBER-1 positive lung cancer tissues and EBER-1 negative tissues was 11.1% and 4.2% respectively,without significant difference. Average area (AA) and integral optical density(IOD) of p53 and Bcl-2 in EBER-1 positive cases were higher than those in negative tissues,with significant difference. AA and IOD of MMP-9 in EBER-1 positive tissues were higher than those in negative tissues,without significant difference(P0.05). Conclusion EBV might play a role in carcinogenesis and metastasis of lung cancer by influencing the expressions of LMP-1,p53,Bcl-2 and MMP-9.