Monomorphism of luteinizing hormone receptor (LHR) gene in buffaloes by restriction fragment length polymorphism

The productivity of buffaloes is commonly affected due to many inherent disorders. Luteinizing hormone (LH) is a glycoprotein hormone of pituitary origin that regulates gonadal function, including steroidogenesis as well as gametogenesis. The present study was undertaken with 203 Murrah / Graded Murrah buffaloes from different locations to investigate the polymorphism of Luteinizing hormone receptor (LHR) gene using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method during June, 2016 to July, 2017. PCR amplification of DNA samples revealed 303 bp product using specific primers and digested with HhaI restriction enzyme. All the tested animals revealed monomorphic pattern at 303 bp and genotyped as TT, which indicates the fixation of T allele and absence of C allele. Consequently, we could not perform association studies with reproductive traits.