Crystallization and preliminary X‐ray analysis of a vanadium‐dependent peroxidase from Ascophyllum nodosum
1996
Peroxidase from the brown alga Ascophyllum nodosum, a non-heme vanadium-dependent haloperoxidase, has been purified to homogeneity and crystallized from ammonium sulfate solutions in a form suitable for X-ray diffraction analysis. The crystals have been grown by the vapour-diffusion technique using the sitting-drop method. X-ray diffraction studies show that the crystals belong to the tetragonal space group P41212 or P43212 with a = b = 114.3 and c = 276.0 A. The crystals diffract to at least 2.4 A resolution.
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