Cloning and sequence analysis of HbERF3 promoter from Hevea brasiliensis

This experiment was designed to study the expression regulation,function in signal transduction and the stress-resistion of HbERF3 gene in Hevea brasiliensis.We designed two specific primers based on the sequence of the ERF3 gene from H.brasiliensis,and a 1 572 bp fragment was cloned from the genomic DNA of H.brasiliensis by GenomeWalking method.Sequence analysis showed that the fragment was the 5’ upstream promoter region of ERF3 gene and it contained typical eukaryotic core promoters and a transcription start site.The analysis of the cis-acting elements showed that the fragment contained some TATA-box,CAAT-box as well as some elements that responsed to hormone and stresses.The cloning and the sequence analysis of the HbERF3 promoter lay the foundation to a further study of the regulation and function of HbERF3.