Effectiveness Of Bacteria Decarboxylase That Is Isolated From Mangrove Waters For Decompose Histidine To Histamine

This research intent to isolate Mangrove's plant bacteria and applying on substance L-histidine becomes histamine on In Vitro's system. Material of research which is Media TSB, aquabidest, aquadest, NaCl, powder L-Histidine Merk SIGMA and sample bacteria of Mangrove's plant Rhizophora Apiculata and Avicennia alba, incubator, autoklaf, resin's column, spektrofluorometer, stirer plate, waterbath. Research method is explorative with parameter which is bacteria Isolation test, biochemical test with Microbact identification system and Application bacteria on L-Histidine with aeration time that is utilized, namely 6 hours, 12 hours, 18 hours, and 24 hours to Test histamine rates. Acquired result 5 isolat bacterias which is Mangrove (Rhizophora Apiculata) there is 3 isolat bacterias which is B (Bacillus megaterium), E( Enterobacter gergoviae) and P (Planococcus citreus) and Mangrove (Avicennia alba) there is 2 isolat bacterias which is N (Nitrococcus sp) and A (Acinetobacter baumanii). Histamine rate N ( Nitrococcus sp) 0,12 mg / kg; 1,19 mg / kg; 3,55 mg / kg; 0,23 mg / kg, A (Acinetobacter baumanii) 6,18 mg / kg; 8,68 mg / kg; 5,31 mg / kg; 4,21 mg / kg and NA( Nitrococcus sp + Acinetobacter baumanii) as big as 2,89 mg / kg; 6,90 mg / kg; 3,26 mg / kg; 2,43 mg / kg. Histamine rate B (Bacillus megaterium) 3,54 mg / kg, 1,30 mg / kg, 3,45 mg / kg, 1,95 mg / kg, E( Enterobacter gergoviae) 6,73 mg / kg, 2,84 mg / kg, 2,78 mg / kg, 0,26 mg / kg, P( Planococcus citreus) 6,04 mg / kg, 7,28 mg / kg, 8,53 mg / kg, 12,55 mg / kg and B + E + P (Bacillus megaterium + Enterobacter Gergoviae + Planococcus Citreus) 1,61 mg / kg, 1,09 mg / kg, 3,33 mg / kg, 3,81 mg / kg. Concluded Nitrococcus sp and Acinetobacter baumannii can not decompose histidine becomes histamine( bacteria Non Decarboxylase) and Planococcus citreus, Bacillus megaterium and Enterobacter gergoviae . can decompose histidin as histamin (Decarboxylase bacteria).