Abstract 191: Immobilized ApoA-I and High Density Lipoproteins are Anti-thrombotic and Inhibit Smooth Muscle Cell Attachment and Proliferation

Aim: The clinical efficacy of current endovascular stents is limited by thrombosis and restenosis. Plasma activated coated stainless steel (PAC) is able to covalently bind proteins to stainless steel (SS) surfaces in their bioactive state. High density lipoproteins (HDL) and its main apolipoprotein constituent apoA-I regulate key biological processes involved in restenosis and thrombosis, highlighting their potential for immobilization on PAC stent surfaces. We hypothesized that covalently bound apoA-I or reconstituted HDL (rHDL) to PAC will retain its biological properties and improve stent patency. Methods and Results: Covalent binding of 125I labelled apoA-I and rHDL was increased on PAC compared to SS (p<0.001). Thrombosis formation under static conditions was assessed by scanning electron microscopy. Thrombi were strikingly reduced on PAC and completely absent on PAC+apoA-I and PAC+rHDL, while extensive thrombi formed on all SS samples at all time points. Thrombus formation under flow conditions usin...