Abstract 1221: Establishment and characterization of a chemoresistant glioblastoma cell line from an Iraqi patient

Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA Glioblastoma multiforme is the most common and aggressive malignant primary brain tumour in humans, and has a very poor prognosis. As it is a well known fact that environmental factors especially radiation exposure is a major risk for glioblastoma multiforme and Iraq had been exposed in a relatively short period to 2 international wars with documented radiation exposure, so this paper might give justification for establishment of a new glioblastoma cell line. This cell line has been established from a cerebral glioblastoma multiforme in a 72-year-old Iraqi man who underwent surgery for an intracranial tumour in 2005. The morphology, growth kinetics, karyotype, immunocytochemistry and angiogenesis factor expression profile of this ANGM5 cell line were studied. The cell line has been grown continuously for more than 200 serial passages in standard culture media presenting no sign of cell senescence. The cultured cells are elongated or multipolar in shape and the population doubling time is 28 hours. The karyotype is complex and the chromosomal number varied between 46 and 48. The cell line is resistant to Temozolomide, cisplatin, vincristine and etoposide. This chemoresistance could be explained by the overexpression of the breast cancer resistance protein (BCRP) which found to be overexpressed by this cell line. Immunocytochemistry also showed that this cell line exhibited several characteristic features of glioblastomas, analysis of glial markers demonstrated that cells are positive for glial fibrillary acidic protein (GFAP), and negative for nestin and calbindin. Protein microarray analysis showed very high production of TIMP2 as well as other factors that are important for the invasiveness and aggressiveness of glioblastoma. This glioblastoma cell line is a useful addition to the cell lines currently available for study of the pathobiology and chemoresistance properties of glioblastoma multiforme as well as for antitumour drug discovery. Citation Format: Ahmed M. Al-Shammari, Ahmed A. Al-Juboory, Asmaa A. Al-Mukhtar, Amal M. Ali, Zaid A. Al-Hili, Nahi Y. Yaseen. Establishment and characterization of a chemoresistant glioblastoma cell line from an Iraqi patient. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1221. doi:10.1158/1538-7445.AM2014-1221