You Should Have the Same Area Counts

Mass spectrometry is a sensitive technique used to detect, identify, and quantify molecules based on their m/z . High-performance liquid chromatography (HPLC)4 is a common separation technique used before analysis of biological samples by LC-MS/MS. LC-MS/MS is preferred over immunoassay for therapeutic drug monitoring of immunosuppressive drugs due to its specificity and sensitivity in detecting analytes in liquid and nonvolatile samples. However, time-consuming sample preparation and interferences resulting from ion suppression are among the disadvantages of LC-MS/MS because they can compromise or invalidate results. Also, although the instruments used are more robust, computer-interfaced, user-friendly, and easier to maintain compared with decades ago (1), their technical complexity leads to difficulties in troubleshooting and can be intimidating to laboratorians. Creating exhaustive system checklists …