[Impact of different emulated acupuncture-needle manipulations on blood pressure and myocardial angiotensin II content in spontaneous hypertension rats].

Objective To observe the effect of different emulated acupuncture-needle manipulations on blood pressure and myocardial angiotensin(Ang) Ⅱ content in spontaneously hypertensive rats(SHR),so as to seek a better acupuncture parameter for clinical treatment of hypertension.Methods Thirty-three SHR were randomly divided into mild-stimulation group(n=8),moderate-stimulation group(n=8),heavy-stimulation group(n=8) and model group(n=9).Additionally,other 8 normal rats were made up of control group.Blood pressure(BP) was determined by using a non-invasive BP detection system.Myocardial Ang Ⅱ content was assayed by radioimmunoassay.The emulated acupuncture-needle twirling angles and frequencies of 144°,75 r/min;255°,111 r/min;and 360°,140 r/min in mild,moderate and heavy manipulations were applied to bilateral "Zusanli"(ST 36) and "Taichong"(LR 3) for 3 min,once daily for 3 weeks,with an interval of 2 days between every two weeks.Results Compared with pre-therapy,BP values of both heavy-stimulation and model groups increased significantly(P0.01);and compared with model group,BP values of mild-stimulation and moderate-stimulation groups decreased significantly after the therapy(P0.05),but were still higher than that of control group(P0.05).In addition,myocardial Ang Ⅱ contents of mild-stimulation,moderate-stimulation,heavy-stimulation and model groups were significantly higher than that of control group(P0.05).No significant differences were found between mild-stimulation and moderate-stimulation groups,and between heavy-stimulation and model groups after therapy in BP,and among mild-stimulation,moderate-stimulation,heavy-stimulation and model groups in myocardial Ang Ⅱ contents(P0.05).Conclusion Mild and moderate manipulations of ST36 and LR3 have apparent influence on BP,but no apparent influence on myocardial Ang Ⅱ content.