Method for regenerating and transforming cotton.
1988
METHODS AND TRANSFORM ALGODON to regenerate. REGENERATION IS PERFORMED cultivating an explant COTTON for a sufficient time to develop calluses, TRANSFERRING SUCH A growth medium, GROWING corns TO DEVELOP CALLOS embryogenic, transferring these to a germination medium and culturing the embryogenic calluses said means UNTIL DEVELOP plantlets from them. THE TRANSFORMATION IS MADE ONE TRYING explant of cotton, Agrobacterium vector, INCUBADNO DURING THE explant 15-200 hours at a temperature of 25-35GC, TRYING explants incubated with medium containing a ANTUBIOTICO CAPABLE OF KILLING Agrobacterium, GROWING FREE explant Agrobacterium and removing calluses ASI TRANSFORMADOS. Cotton plants obtained are useful for their resistance to antibiotics NORMALLY GROWTH INHIBITORS their cells.
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