Determination of culture medium composition for maximizing the biomass production of Pseudomonas stutzeri
2017
In the recent years, extensive studies have been conducted on the application
of bioremediation in order to prevent and solve the global problem of nitrate
accumulation especially in water and soil. In the studies related to the
biological denitrification, Pseduomonas stutzeri is usually used as a model
microorganism due to its ability of performing a complete reduction of
nitrate. The aim of this work is to establish a culture medium at a
laboratory level for maximizing the biomass production of Pseduomonas
stutzeri ATCC 17588 and Pseudomonas stutzeri D1, previously isolated from the
Danube (Novi Sad, Serbia) by using a Box-Bhenken experimental design and
response surface methodology. The selected independent variables (+, 0, -
levels) were: glucose (0, 1 and 2 g/L), KNO3 (1, 2 and 3 g/L), and peptone
(0.4, 2 and 4 g/L), while the number of viable cells (log/mL) was chosen as a
dependent factor (response). Based on the obtained results, it can be noticed
that the maximum number of P. stutzeri ATCC 17588 viable cells is achieved
when the initial content of glucose, KNO3 and peptone in the medium are 0
g/L, 2 g/L and 4 g/L, respectively. In the case of P. stutzeri D1, the most
appropriate media for enhancing the biomass production consists of glucose (1
g/L), KNO3 (3 g/L) and peptone (4 g/L). Тhe obtained results can be used for
further technoeconomic analysis and scale up of the P. stutzeri biomass
production.
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