Solid-aqueous phase partitioning of radionuclides by complexing compounds excreted by subsurface bacteria

Radionuclides are present in numerous aerobic and anaerobic subsurface environments due to nuclear weapons testing, leakage from process and storage facilities, and discharge of radioactive waste. The partitioning of radionuclides between liquid and solid phases by complexing compounds excreted by subsurface bacteria was studied. The solid–aqueous phase partitioning of pico- to submicromolar amounts of 59Fe, 147Pm, 234Th, and 241Am was analyzed in the presence of quartz sand and exudates from three species of subsurface bacteria: Pseudomonas fluorescens, Pseudomonas stutzeri, and Shewanella putrefaciens. All were grown under aerobic conditions, and P. stutzeri and S. putrefaciens were grown under anaerobic conditions as well. The supernatants of the aerobic and anaerobic cultures were collected and radionuclide was added. Quartz sand, with a Brunauer, Emmett, and Teller (BET) surface area of 0.1 m2 g–1, was added to the supernatant radionuclide mix, and the pH was adjusted to approximately 8. After centri...