Sterilizing effect and residual bacteria of four different methods for sterilizing simple breathing vesicles

Objective To study the effects of 4 different methods for disinfection of simple breathing vesicles and microbial residue. Methods The disinfection tests were divided into 4 groups: G1 group (43 cases) with 500 mg/L chlorine dioxide spray, G2 group (28 cases) with alcohol spray, G3 group (47 cases) with 50 mg/L trichloro isocyanuric acid (TCCA) immersion, and G4 group (46 cases) with 50 mg/L chlorine dioxide solution immersion. Afrer 30 min of dsinfection, each group was examined by bacterial culture and colony count. The residual bacteria were identified and typed. Results The 4 methods showed significant differences in bacterial colony count (P0.001). The rate of bacterial residue was 0% in G1 group, 53.6% in G2 group, 27.7% in G3 group, and 21.7% in G4 group, showing significant differences between the 4 groups (P0.001). The residual bacteria included antibiotic- resistant common opportunistic pathogen such as Pseudomonas aeruginosa, Acinetobacter baumannii and Staphylococcus haemolytic. Conclusions Disinfection with 500 mg/L chlorine dioxide spray is the best for simple breathing vesicles. Prolonged immersion in TCCA may lead to the growth of drug-resistant pathogens in the breathing vesicles.