Syzygium cumini leaf extract inhibits LDL oxidation, but does not protect the liproprotein from glycation

Abstract Etnopharmacological relevance Syzygium cumini (L.) Skeels is a plant widely used in folk medicine to treat diabetes mellitus (DM). The tea from its leaves is frequently used by diabetics for lowering hyperglycemia. There is a close relationship between DM and atherosclerosis, a chronic immuno-inflammatory disease, were the early stages encompass oxidative and glycative modifications in the structure of low density lipoprotein (LDL). Aim of this study To investigate the potential protective effects of aqueous-leaf extract from Syzygium cumini ( S.c Ext) against CuSO 4 -induced oxidation and methylglyoxal (MG)-induced glycation of human LDL in vitro . Materials and methods LDL oxidative changes were evaluated by measuring conjugated dienes (CD) formation, thiobarbituric acid reactive substances (TBARS) levels, quenching of tryptophan (Trp) fluorescence and structural modifications in LDL particle. In LDL glycated by MG (glyLDL), we determined the levels of fluorescent advanced glycation end products (AGEs) and mobility by agarose gel electrophoresis. Results S.c Ext blocked oxidative events induced by CuSO 4 in human LDL, plasma and serum. Fourier transform infrared spectroscopy (FT-IR) revealed that specific regions of apoB100 were oxidized by CuSO 4 in human LDL and that S.cExt reduced these oxidations. Unlike, the increased AGEs levels and eletrophoretic mobility observed in LDL MG-glycated were not modified by S.c Ext. Conclusion The findings herein indicate that S.c Ext could be tested in atherogenesis models as potential protective agent against LDL oxidation.