GW24-e2369 Aliskiren inhibits proliferation of vascular smooth muscle cells induced by platelet-derived growth factor-BB via extracellular regulated protein kinases signal pathway

Objectives To identify the effect of direct renin inhibitor aliskiren on proliferation of vascular smooth muscle cells (VSMCs) induced by PDGF-BB, and study the mechanism of this effect. Methods The rats thoracic aorta smooth muscle cells (A10 cells) were used in the study, the effects of different concentrations of aliskiren and PDGF-BB as well as different stimulus durations of 10 μmol/L aliskiren and 20 ng/mL PDGF-BB stimulated on VSMCs proliferation was observed by [3H]-TdR; ERK, p-ERK and β-actin were assessed by immunoblotting. Results PDGF-BB could induce A10 cell proliferation, and the ratio of cell proliferation treated with 20 ng/mL PDGF-BB was (145.50 ± 10.51) % (P Conclusions Aliskiren suppressed PDGF-BB-induced proliferation of VSMCs, via ERK1/2 signal pathway.