Establishment of an insecticide resistance monitoring protocol based on the residual contact vial bioassay for Frankliniella occidentalis

Abstract A modified residual contact vial bioassay (RCV) in which a small aliquot (1 μl) of water was supplemented to minimize control mortality was established as a rapid insecticide resistance monitoring tool for the western flower thrips, Frankliniella occidentalis . Based on the RCV scheme, diagnostic doses for seven insecticides that are widely used for F. occidentalis control were determined at 8 h post-treatment using a susceptible RDA strain. The diagnostic doses for five insecticides (chlorfenapyr, acrinathrin, spinosad, emamectin benzoate and thiamethoxam) were in the range of 0.03 to 0.42 μg − 1  cm 2 and were readily applicable for the detection of resistance levels. In the case of the remaining two insecticides (omethoate and imidacloprid), however, the estimated diagnostic doses were too high (3.28 and 12.4 μg − 1  cm 2 , respectively) to form a viscous film over the inner wall of the treated vial, thereby limiting their use for resistance detection. Thus, the performance of RCV in detecting resistance to the five insecticides was evaluated for five local populations of F. occidentalis . The RDA strain exhibited 100% mortality to all insecticides tested, whereas field populations collected from horticultural glass houses generally showed remarkably reduced mortality ( F. occidentalis .