The effect of ATP on the CD spectrum of membrane fraction from oxyntic cells

Abstract A vesicular preparation derived from acid-secreting cells of dog gastric mucosa was studied in a Cary 60 modified for simultaneous CD and absorbance measurements. Significant changes in the spectrum were obtained on addition of MgATP, but not MgAMP, which were only partly due to changes in scattering, etc . Thus ATP may induce conformational changes in the protein constituents of the vesicle membrane.