The complete structure of the core carbohydrate backbone from the LPS of marine halophilic bacterium Pseudoalteromonas carrageenovora type strain IAM 12662T

Abstract The complete novel structure of the components of the core oligosaccharide fraction from the LOS of the halophilic marine bacterium Pseudoalteromonas carrageenovora was characterized. The fully de-acylated lipooligosaccharide was studied by means of compositional analysis, matrix-assisted laser desorption/ionization mass spectrometry and complete 1 H and 13 C and 31 P NMR spectroscopy. The core oligosaccharide is composed by a mixture of species differing for the length of the sugar chain and the phosphorylation pattern: All sugars are d -pyranoses. Hep is l - glycero - d - manno -heptose, Kdo is 3-deoxy- d - manno -oct-2-ulosonic acid, P is phosphate, residues and substituents in italic are not stoichiometrically linked.