Effects of Hydrogen Bonding on Internal Conversion of GFP-like Chromophores. I. The para-Amino Systems
2013
To rationalize the efficient quenching of the fluorescence and the Z → E photoisomerization of m-ABDI, the meta-amino analogue of the green fluorescent protein (GFP) chromophore, in protic solvents, the femtosecond time-resolved fluorescence and transient infrared (TRIR) spectra of m-ABDI in CD3CN, CH3OH, and CD3OD are determined. For solutions in CD3CN, the fluorescence decay lifetime is ∼7.9 ns and IR absorption lines near 1513, 1531, 1557, and 1613 cm–1 of m-ABDI in its electronically excited state were observed with a decay time >5 ns. For solutions in CH3OH, the fluorescence decay is double exponential with time constants of ∼16 and 62 ps. In addition to IR absorption lines of m-ABDI in its electronically excited state with a decay time of ∼16 ps, new features near 1513, 1532, 1554, and 1592 cm–1 were observed to have a rise time of ∼19 ps and a decay constant of ∼58 ps, indicating formation of an intermediate. The assignments for the IR spectra of the ground and excited states were assisted with DFT...
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