β-citronellol alters cell surface properties of Candida albicans to influence pathogenicity related traits

The pathogenicity of Candida albicans, an opportunistic human fungal pathogen, is attributed to several virulence factors. beta-citronellol is a monoterpenoid present in several plant essential oils. The present study explores the antifungal potential and mode of action of beta-citronellol against C. albicans ATCC 90028 (standard), C. albicans D-27 (FLC-sensitive), and C. albicans S-1 (FLC-resistant). Anti-Candida potential was studied by performing MIC, MFC, growth curves, disc diffusion, spot assay, and WST1 cytotoxic assay. Morphological transition was monitored microscopically in both solid and liquid hyphae inducing media. beta-citronellol inhibits yeast to hyphal transition in both liquid and solid hyphae inducing media. It had a significant inhibitory effect on biofilm formation and secretion of extracellular proteinases and phospholipases. We showed that it has an adverse effect on membrane ergosterol levels and modulates expression of related ERG genes. Expression profiles of selected genes associated with C. albicans pathogenicity displayed reduced expression in treated cells. This work suggests that beta-citronellol inhibits morphological transition in C. albicans and decreases the secretion of hydrolytic enzymes involved in the early stage of infection as well as modulates the expression of associated genes. Pleiotropic phenotype shown by beta-citronellol treated Candida cells suggests various modes of action. Further studies will assess the clinical application of beta-citronellol in the treatment of fungal infections.