Purification of Newcastle disease virus by chromatography on controlled-pore glass bead column.

: Newcastle disease virus (NDV) purified by sucrose density gradient centrifugation was chromatographed on a controlled-pore glass (CPG) bead column. By this procedure, the contaminating ribonuclease activity was reduced by 94-96% and the specific viral haemagglutinating activity increased from 3-to 5-fold. Purified NDV moved in two fractions in free electrophoresis.