[Determination of Flavonoids in Cycas revoluta Leaves by Chemiluminescence-Flow Injection Analysis Method].

Objective: To detect flavonoids from Cycas revoluta leaves by means of Chemiluminescence-Flow Injection Analysis( CL-FIA). Methods: Under alkaline condition,a CL-FIA method was established to determine flavonoids from leaves of Cycas revoluta on the basis of inhibiting effect of flavonoids to the Luminol-H2O2-Cu2 +chemiluminescence system and the reversed flow injection technique. Results: In the range of 2. 0 × 10- 6~ 1. 0 × 10- 3mg / m L,the decrease of CL intensity was correlated with flavonoids concentration while the detection limit was 0. 0265 μg / m L. Under the optimized conditions,the flavonoids of Cycas revoluta leaves were detected with its average rate reaching 1. 61% and RSD 1. 32%. Conclusion: Through the interference test and compared with the data of CL-FIA and UV,it is concluded that CL-FIA can be used in the analysis and detection of flavonoids from Cycas revoluta leaves.