Comparison of biopersistence of experimental glass fibres in the lung and peritoneal cavity

Abstract Thirty three female Fischer-344 rats were intra-peritoneally (IP) injected with 5 mg of an experimental glass fibre designated X7753. This fibre type had an in vitro dissolution rate of 600 ng cm −2 h −1 . Groups of three rats were killed at various times up to one year after injection. The diaphragm and any fibre nodules were removed from the carcass and separately digested using hypochlorite solution, to recover the fibres. The number and morphometry of the fibres was measured using phase contrast optical microscopy (PCOM) and semi-automatic image analysis. The data obtained were compared to the previous studies of the durability of the X7753 fibres in the lung.