Changes in the alkaline phosphatase activity and properties of the blood serum in sheep with experimental liver disease

: An acute liver injury was provoked in 13 sheep by using tetrachlormethane. The study traced out the changes in the activity and properties of alkaline phosphatase in the blood serum. It was proved that the total activity of the enzyme increased from an average of 60.7 +/- 7.8 UI to an average of 104.3 +/- 14.8 UI on the 72d hour of the experiment, and then it decreased. In the first 24-48 hours after the treatment with tetrachlormethane the inhibition of the enzyme increased by the use of 1-homoarginine (on an average from 60.0 to 65.6%), whereas the use of 1-phenylalanine put down inhibition (on an average from 36.9 to 31.3%). In the next days the inhibition decreased due to 1-homoarginine (up to an average of 48.5%) and increased due to 1-phenylalanine. It was proved that in the initial phase of the poisoning in the blood serum of sheep the contents of the liver isoenzyme of the alkaline phosphatase increased, whereas in the second phase it was the contents of the liver isoenzyme of the latter that increased.