[IL-10 suppresses inflammatory granuloma uptake of 18F-FDG in rats].

Objective: To evaluate the effectiveness of IL-10 in suppressing 18F-FDG uptake in the inflammatory granuloma of SD rats. Methods: Eight SD rats were killed,and their blood was collected sterily.After centrifugion,the white blood cells were incubated in PRMI 1640 for 3 days.Then each culture flask of white blood cells was divided into two equal parts.To one group was added 0.2 mL IL-10 solution(0.1 mg/mL);to the control was added with 0.2 mL of 0.9% sodium chloride solution.All cells were then incubated for 120 minutes at 37 ℃,after which 18F-FDG(1.85 MBq) was added.Sixty minutes later,the cells were washed twice with PBS and the extent of uptake 18F-FDG determined.In vivo,an inflammatory granuloma was produced by hypodermic injection of rats with a mixture of Freund's complete adjuvant,bovine serum albumin and talcum powder.Each rat was maintained for 8 weeks.Imaging of the inflammatory granulomas was performed using the 18F-FDG signal.IL-10 was injected into SD rats at 10 μg/kg of body weight.Sixty minutes later,7.4 MBq of 18F-FDG were injected,and,after a further 60 minutes,the rats underwent a PET-CT scan.The region of interest(ROI) of the inflammatory granuloma was delineated and the standard uptake value(SUV) calculated.A second PET-CT scan was done without IL-10 on the next day.The granulomatous tissue underwent pathological examination. Results: In the intro test,the with blood cell uptaking ratio of 18F-FDG was(50.3±6.7)% without IL-10,and(34.6±3.5)% with IL-10(t=8.9,P0.01).IL-10 suppressed the rat white blood cell uptaking 18F-FDG.In the PET-CT scan,the SUV of ROI on inflammatory granuloma was 1.7±0.4 with IL-10 and 2.1±0.3 without IL-10(t=20.6,P0.01).IL-10 suppressed the inflammatory granuloma uptaking 18F-FDG. Conclusion: IL-10 can suppress the inflammatory granuloma of SD rats uptaking 18F-FDG.