Isolation and phenotypic and genotypic characterization of the potential probiotic strains of Lactobacillus from the Iranian population.
2021
Among different causes of inflammatory bowel disease (IBD), the imbalance of the gut microbiome (dysbiosis) is one of the main reasons for the development of the disease. Probiotics are live microorganisms that can maintain gut microbiota by different mechanisms. We aimed to isolate and characterize the potential probiotic strains of Lactobacillus from the Iranian population. This cross-sectional study was conducted on faecal samples of 83 volunteer individuals living in Guilan Province, North Iran. The primary identification of Lactobacillus strains was performed by standard microbiological tests and confirmed by amplification of 16s rRNA specific primers. The acid and bile salt tolerance were assessed for all recovered strains. Also, the presence of 3 bacteriocins encoding genes was investigated by the PCR method. Totally, 42 samples were positive for Lactobacillus species. Acid and bile resistance assay showed that 67% and 33% of strains were resistant to acid and bile salt stress, respectively. Therefore, we found out that 28% of our Lactobacillus strains have the ability for resistance to acid and bile conditions. PCR results revealed that the prevalence of gassericin A, plantaricin S, lactacin bacteriocin genes were 16.6%, 12%, and 9.5%, respectively. Meanwhile, 5 out of 12 Lactobacillus strains that were resistant to acid and bile conditions contained one of the gassericin or plantaricin bacteriocins. We isolated 42 potential probiotic strains of Lactobacillus, of which the results of 5 strains were more promising and can be considered as potential probiotics sources for future functional products.
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