New Supercritical Fluid Extraction Treatment Method for Determination of Tripterine in Tripterygium wilfordii Hook. F

Abstract A new supercritical fluid extraction (SFE) pretreatment method for determination of tripterine in Tripterygium wilfordii Hook. F with high performance liquid chromatography is proposed in this paper. The optimized conditions of SFE were set as follows: n‐butanol was selected as cosolvent and the ratio of n‐butanol to the material was 1.0 mL/g material; the extraction temperature and pressure were 40°C and 35 MPa, respectively. The static and dynamic extraction times were both 15 min. Recovery of tripterine from SFE varied from 90.5 to 103.2% for samples with different content levels. During the validation of the method, the linearity and precision, stability, limit of detection and quantification were also determined, all of which satisfied the analytical requirements. Comparison of SFE with conventional soxhlet extraction shows SFE is more efficient in the extraction of tripterine, as it needs less organic solvent and extraction time. Besides, it does not need a further cleanup procedure before ...