Study on the Effect of Extracts from the Leaves of Phyllanthus emblica on Immune Function of Mice

Objective:To explore the effect of extracts from the leaves of Phyllanthus emblica(PLFs)on the immune function of mice.Methods:70 Kunming mice were choosed to conduct the acute toxicity test of PLFs.The mice were randomly divided into four groups:PLFs high-dosage group,mid-dosage group,low-dosage group and control group.The high,mid,low-dosage groups were treated with PLFs 1.982,0.991 and 0.496 g/kg respectively per day.The same volume of double distilled water was given to the control group.All by intragastric administration for 7 d.The animals were killed and indexes of thymus and spleen were calculated.The expurgation index K and phagocyte index α were detected after the mice being injected with a dilute India ink through caudal vein.In addition,prepared spleen cells conventionally,the activity of Natural Killer cells was measured and the proliferation of T and B cells were detected.The effect of the extracts on serum hemolysin was detected after the SRBC was injected into the enterocoelia.Results:The LD50 of PLFs was 9.911 g/kg.Compared with the control group,the indexes of thymus and spleen in the treatment groups had no markedly difference(P0.05).The high-and mid-dosage groups could obviously improve the expurgation index K(P0.05),phagocyte index α(P0.05) and NK cell activity(P0.05).Conclusion:The extracts from Phyllanthus emblica leaves can promote nonspecific immunity immune function in mice.